Polymerase Stalling during Replication, Transcription and Translation

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Polymerase Stalling during Replication, Transcription and Translation

Replication, transcription, and translation stress all lead to stalling of their respective polymerases (DNA polymerase, RNA polymerase, and the ribosome), and the cell must respond to these events in order to preserve macromolecular integrity. In response to replication stress such as DNA damage, the cell activates a checkpoint and promotes repair or bypass at the lesion. Transcriptional stres...

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Theoretical analysis of transcription process with polymerase stalling.

Experimental evidence shows that in gene transcription RNA polymerase has the possibility to be stalled at a certain position of the transcription template. This may be due to the template damage or protein barriers. Once stalled, polymerase may backtrack along the template to the previous nucleotide to wait for the repair of the damaged site, simply bypass the barrier or damaged site and conse...

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Structural basis of error-prone replication and stalling at a thymine base by human DNA polymerase

Thank you for submitting your manuscript for consideration by the EMBO Journal. It has now been seen by three referees whose comments are enclosed. As you will see, all three referees show interest in your work and are broadly in favour of publication. However, all three raise criticisms that would need to be addressed in a revised version of the manuscript before we could consider publication....

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Replication fork stalling at natural impediments.

Accurate and complete replication of the genome in every cell division is a prerequisite of genomic stability. Thus, both prokaryotic and eukaryotic replication forks are extremely precise and robust molecular machines that have evolved to be up to the task. However, it has recently become clear that the replication fork is more of a hurdler than a runner: it must overcome various obstacles pre...

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Comparison of pausing during transcription and replication.

Pausing during the transcription of MDV-1 cDNA by Escherichia coli RNA polymerase was compared with pausing during the replication of MDV-1 RNA by Q beta replicase. MDV-1 RNA is able to form many strong hairpin structures, and Q beta replicase pauses after the synthesis of each [Mills et al. (1978) Cell 15, 541-550]. Although the transcripts were virtually identical to MDV-1 RNA, the locations ...

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ژورنال

عنوان ژورنال: Current Biology

سال: 2014

ISSN: 0960-9822

DOI: 10.1016/j.cub.2014.03.060